DCI

CAS 1122-28-7, Cat. No EN300-77565

Activator for DNA and RNA Fragment Synthesis

1H-Imidazole-4,5-dicarbonitrile (DCI) is a popular activator for constructing the nucleoside phosphoramidite. It is a solid soluble in acetone, methanol, ethanol, and DMF, but practically insoluble in water. The procedure for this activator is simple and does not require heating. After the reaction, which usually takes one night, chromatography can easily remove the DCI from the product.

Synonyms: Imidazole-4,5-dicarbonitrile (6CI, 7CI, 8CI); 2H-Imidazole-4,5-dicarbonitrile; 4,5-Dicyanoimidazole

Selected publication

1. 4,5-Dicyanoimidazole.

Sebesta D. P.; Vagle K.; Rachiero G. P.; Rogers R. D. Encyclopedia of Reagents for Organic Synthesis 2018, 1–7. DOI: 10.1002/047084289X.rn00166

2. Efficient Activation of Nucleoside Phosphoramidites with 4,5- Dicyanoimidazole during Oligonucleotide Synthesis.

Vargeese C. Nucleic Acids Res 1998, 26 (4), 1046–1050. DOI: 10.1093/nar/26.4.1046