Cyanoethyl tetraisopropylphosphorodiamidite

CAS 102691-36-1, Cat. No EN300-239576

Reagent for phosphitylation of DNA or RNA fragments

2-Cyanoethyl tetraisopropylphosphorodiamidite is a phosphitylation reagent for DNA and RNA fragment synthesis that became very important in this field of study. It is a colorless liquid that is shelf-stable well well-soluble in most organic solvents. The reagent is stable for hydrolysis and offers an easy procedure that requires only an activator, such as (EN300-77565), and no heating. After the reaction, which usually takes one night, chromatography can easily purify the product.

Synonyms: bis(N,N-diisopropylamino)-2-cyanoethoxyphosphine; (2-cyanoethoxy) bis(N,N-diisopropylamino)- phosphine, 2-cyanoethyl N,N,N,N -tetraisopropylphosphordiamidite

Selected publication

1. Highly Efficient Chemical Synthesis of 2′-O-Methyloligoribonucleotides and Tetrabiotinylated Derivatives; Novel Probes That Are Resistant to Degradation by RNA or DNA Specific Nucleases.

Sproat B. S.; Lamond A. I.; Beijer B.; Neuner P.; Ryder U. Nucleic Acids Res 1989, 17 (9), 3373–3386. DOI: 10.1093/nar/17.9.3373

2. 2-Cyanoethyl Tetraisopropylphosphorodiamidite.

Beaucage S. L. Encyclopedia of Reagents for Organic Synthesis 2003. DOI: 10.1002/047084289X.rn00312